pro il1β  (R&D Systems)

Journal: Molecules

Article Title: Walnut Kernel Oil and Defatted Extracts Enhance Mesenchymal Stem Cell Stemness and Delay Senescence

doi: 10.3390/molecules28176281

Figure Lengend Snippet: WKO and WKD extracts protect rat BM-MSCs against inflammation induced by LPS. Effect of the two extracts on the levels of pro-inflammatory cytokines TNFα, IL1β, and NF-kB as measured by ELISA. Values are presented as mean ± SEM, n = 3. Letters a–d denote significant differences between the four groups at p < 0.05. Each group was compared against every other group. DMSO, vehicle-treated control cells; LPS, lipopolysaccharides-treated group; WKO, walnut kernel oil extract-treated cells; WKD, walnut kernel defatted extract-treated cells.

Article Snippet: However, the levels of pro-inflammatory cytokines IL1β (MyBioSource, Cat # MBS825017; OD = 450 nm; CV of intra-assay precision = 5.9%; CV of inter-assay precision = 5.0%), TNFα (MyBioSource, Cat # MBS2507393; OD = 450 nm; CV of intra-assay precision = 6.5%; CV of inter-assay precision = 5.5%), and NF-kB (MyBioSource, Cat # MBS453975; OD = 450 nm; CV of intra-assay precision = 10%; CV of inter-assay precision = 12%) were detected in conditioned media of BM-MSCs after induction of their release by LPS using specific rat ELISA kits as detailed in the manufacturer’s guidelines.

Techniques: Enzyme-linked Immunosorbent Assay, Control